ISSN : 1300-2945
eISSN : 1308-9889
Abstract - Effects Of Thymoquinone On Oxidative Stress In The Testicular Tissue Of Reserpinized Rats
Dilek Kuzay

Dilek Kuzay, Department of Physiology, Faculty of Medicine, Ahi Evran University, 40100 Kırşehir, Turkey              

e-mail: dilekkuzay@gmail.com

Abstract

Objective: The objective of the study was to  research the oxidant and antioxidant activity of Thymoquinone (TQ) in

the testicular tissue of Reserpinized rats. 

Methods: Eighteen rats were divided into three groups and each group had six animals: 1) Control (C) group: Received

ip. 1% Tween 80; 2) Reserpine (R) group: Received Reserpine; 3) Reserpine + TQ (R+T) group: Received Reserpine

and TQ. Reserpine was injected intraperitoneally 0.2 mg/kg and TQ was administered intragastrically 10 mg/kg once

daily. The rats were treated for 14 consecutive days. At the end of the study, total nitric oxide (NOx) levels,

malondialdehyde (MDA) levels and glutathione (GSH) levels in the testicular tissue were examined.

Results: A statistically significant increase was observed in the NOx levels (p=0.001) and MDA levels of testicular

tissue in the R group (p<0.001). A decrease was observed in the GSH levels (p<0.001). It was found out that there was

a decrease in NOx and MDA levels while there was an increase in GSH levels in the R+T group (p<0.001). 

Conclusion: Reserpine caused an increase in oxidative stress and a decrease in antioxidant capacity in testicular

tissue. TQ yielded recovery of Reserpine induced toxicity by decreasing oxidative stress markers and increasing

antioxidant capacity.

 

Keywords: Reserpine; Oxidative stress; Rats; Testis; Thymoquinone

 

Reserpinize Sıçanların Testis Dokusunda Oksidatif Stres Üzerine Thymoquinone'nin Etkisi

                  

Öz

Amaç: Çalışma Reserpinize sıçanların testis dokusunda Thymoquinone (TQ)’ nun oksidan ve antioksidan etkinliğini

araştırmak amacıyla yapıldı.

Yöntemler: 18 adet Wistar Albino sıçan her grupta 6 sıçan olacak şekilde 3 eşit gruba ayrıldı. Kontrol(K) grubu:

intraperitoneal %1 Tween 80 verildi. Reserpin(R): Reserpine verildi. Reserpine+TQ (R+T): Reserpine ve TQ verildi.

Reserpin intraperitoneal 0.2 mg/kg enjekte edildi ve TQ intragastrik 10 mg/kg günde bir kere verildi. Deney ardışık

14 gün sürdü. Çalışmanın sonunda testis dokusunda toplam nitrik oksit (NOx), malondilaldehit (MDA) ve glutatyon

(GSH) düzeyleri bakıldı.

Bulgular: R grubunda testis dokusunun NOx düzeylerinde (p=0.001) ve MDA düzeylerinde istatistiksel olarak önemli

bir artış oldu (p<0.001). GSH düzeylerinde ise azalma oldu (p<0.001). R+T grubunun NOx ve MDA düzeylerinde

azalma, GSH düzeylerinde ise artış olduğu belirlendi (p<0.001).

Sonuç: Reserpine testis dokuda oksidatif stresi arttırdı ve antioksidan kapasiteyi azalttı. TQ tedavisi oksidatif stres

markırlarını azaltıp, antioksidan kapasiteyi artırarak Reserpine ile indüklenen toksisitede iyileşme sağladı.

 

Anahtar kelimeler: Rezerpin, Oksidatif stres, Sıçan, Testis, Timokinon.

 

Dicle Med J  2019;46 (4): 831-837

Doi: 10.5798/dicletip.661402

Volume 46, Number 4 (2019)